Macadamia Nut allergy is an increasing concern since allergies to tree nuts and seeds tend to be of an extremely severe nature, causing life-threatening and sometimes fatal reactions. The seeds of three macadamia trees (Macadamia integrifolia, M. ternifolia and M. tetraphylla) are valuable food crops with a protein fraction of 8%. People allergic to macadamia nut must strictly avoid the consumption of food containing macadamia nut. Cross contamination during the production process often occurs and macadamia nut residues in food cannot be excluded. Therefore sensitive detection methods for macadamia nut in food are required.